soluble leishmanin as an ideal reagent for skin testing in human leishmaniasis

نویسندگان

محمد حسین علی محمدیان

mohammad hossein alimohammadian زهرا فریبا کجوری

zahra fariba kojori هایده دارابی

haiedeh darabi شیرین ملک زاده

shirin malekzadeh محسن یوسف بیگ

چکیده

delayed-type hypersensitivity reaction measured by skin testing, is an important tool for evaluation of cellular immune response in leishmaniasis and has been used recently as an indication of exposure to infection. skin testing in leishmania infection needs a standard reagent with high specificity, sen sitivity, and potency. in the present work, a soluble leishmanin, and three whole parasite prepara tions from leishmania major, i.e. thimerosal, phenol, and autoclaved leishmanins, were prepared un der standard conditions. these antigens were evaluated and compared in different foci of leishmani asis. the sensitivity and potency of reagents were tested in both recovered cases and patients with ac tive ulcer in two areas endemic to urban and rural leishmaniasis. data obtained showed that, the sensitivity of thimerosal, phenol, and soluble leishmanins are almost equal, but are higher than auto claved one. moreover, the potency of soluble leishmanin was also proved to be higher than other reagents. the purity of soluble leishmanin is higher than other three particulate preparations, and it lacks the majority of membrane antigens. as a result this reagent can be used as a standard and ideal antigen for skin testing in human leishmaniasis.

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Soluble Leishmanin as an Ideal Reagent for Skin Testing in Human Leishmaniasis

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عنوان ژورنال:
iranian biomedical journal

جلد ۱، شماره ۴، صفحات ۳۹-۴۷

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